Javascript yakadzimwa mubrowser yako parizvino.Kana javascript yakadzimwa, mamwe mabasa ewebhusaiti ino haazoshande.
Nyoresa yako chaiyo ruzivo uye chaiyo mishonga yekufarira, uye isu tichafananidza ruzivo rwaunopa nezvinyorwa mune yedu yakakura dhatabhesi uye tokutumira iwe kopi yePDF kuburikidza neemail munguva yakakodzera.
Dzora kufamba kwemagnetic iron oxide nanoparticles kune yakanangwa kuendesa kwe cytostatics
Munyori Toropova Y, Korolev D, Istomina M, Shulmeyster G, Petukhov A, Mishanin V, Gorshkov A, Podyacheva E, Gareev K, Bagrov A, Demidov O
Yana Toropova,1 Dmitry Korolev,1 Maria Istomina,1,2 Galina Shulmeyster,1 Alexey Petukhov,1,3 Vladimir Mishanin,1 Andrey Gorshkov,4 Ekaterina Podyacheva,1 Kamil Gareev,2 Alexei Bagrov,5 Oleg Demidov 6,71Almazov National Medical Research Center yeBazi reHutano reRussian Federation, St. Petersburg, 197341, Russian Federation;2 St. Petersburg Electrotechnical University "LETI", St. Petersburg, 197376, Russian Federation;3 Center for Personalized Medicine, Almazov State Medical Research Center, Ministry of Health yeRussian Federation, St. Petersburg, 197341, Russia Federation;4FSBI "Influenza Research Institute yakatumidzwa zita reAA Smorodintsev" Ministry of Health yeRussian Federation, St. Petersburg, Russian Federation;5 Sechenov Institute of Evolutionary Physiology uye Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation;6 RAS Institute of Cytology, St. Petersburg, 194064, Russian Federation;7INSERM U1231, Faculty of Medicine uye Pharmacy, Bourgogne-Franche Comté University of Dijon, France Kukurukurirana: Yana ToropovaAlmazov National Medical Research Center, Ministry of Health of the Russian Federation, Saint-Petersburg, 197341, Russian Federation Tel +7 981 952694800 952694800 9 Email [email yakadzivirirwa] Mhedziso: Nzira inovimbisa yedambudziko re cytostatic toxicity ndeye kushandiswa kwemagineti nanoparticles (MNP) kune yakanangwa yekuendesa zvinodhaka.Chinangwa: Kushandisa masvomhu kuona akanakisa hunhu hwemagineti inodzora MNPs mu vivo, uye kuongorora kushanda kwemagnetron kuendesa kweMNPs kune mbeva tumors in vitro uye in vivo.(MNPs-ICG) inoshandiswa.In vivo luminescence intensity zvidzidzo zvakaitwa mumabundu mbeva, iine uye isina magineti panzvimbo yekufarira.Zvidzidzo izvi zvakaitwa pane hydrodynamic scaffold yakagadzirwa neInstitute of Experimental Medicine yeAlmazov State Medical Research Center yeRussia Ministry of Health.Mhedzisiro: Kushandiswa kweneodymium magineti kwakasimudzira kuunganidzwa kwakasarudzika kweMNP.Imwe miniti mushure mekutonga kweMNPs-ICG kune mbeva dzinotakura bundu, MNPs-ICG inonyanya kuunganidza muchiropa.Mukusavapo uye kuvapo kwemagnetic field, izvi zvinoratidza nzira yayo yemagetsi.Kunyange zvazvo kuwedzera kwe fluorescence mubundu kwakaonekwa muhupo hwemagineti, simba re fluorescence muchiropa chemhuka harina kuchinja nekufamba kwenguva.Mhedziso: Iyi mhando yeMNP, yakasanganiswa neyakaverengerwa magineti simba, inogona kuve hwaro hwekuvandudzwa kweiyo magineti inodzorwa kuendesa cytostatic mishonga kune bundu tishu.Keywords: fluorescence kuongororwa, indocyanine, iron oxide nanoparticles, magnetron kuburitsa cytostatics, bundu rinonangwa.
Zvirwere zvemamota ndicho chimwe chezvikonzero zvikuru zvekufa kwevanhu pasi rose.Panguva imwecheteyo, masimba ekuwedzera kwezvirwere uye kufa kwezvirwere zvebundu zvichiripo.1 The chemotherapy inoshandiswa nhasi ichiri imwe yekurapa kukuru kwemamota akasiyana.Panguva imwecheteyo, kukudziridzwa kwenzira dzekudzikisa systemic toxicity ye cytostatics ichiri yakakosha.Nzira inovimbisa yekugadzirisa dambudziko rayo rehupfu ndeye kushandisa nano-scale vatakuri kunanga nzira dzekuendesa zvinodhaka, izvo zvinogona kupa kuunganidzwa kwemuno kwezvinodhaka mumatumbu ematumbu pasina kuwedzera kuunganidzwa kwavo munhengo dzine hutano uye matishu.concentration.2 Iyi nzira inoita kuti zvikwanise kuvandudza mashandiro uye kunanga kwemishonga yechemotherapeutic pa tumor tissues, uku ichideredza systemic toxicity.
Pakati peakasiyana nanoparticles akatariswa kuendeswa kwakanangwa kweiyo cytostatic agents, magineti nanoparticles (MNPs) anonyanya kufarira nekuda kweakasarudzika makemikari, zvipenyu, uye magineti zvimiro, izvo zvinogonesa kugona kwavo kwakasiyana.Naizvozvo, magineti nanoparticles anogona kushandiswa seanodziya sisitimu yekurapa mapundu ane hyperthermia (magnetic hyperthermia).Iwo anogona zvakare kushandiswa seane diagnostic agents (magnetic resonance diagnosis).3-5 Uchishandisa hunhu uhwu, hwakasanganiswa pamwe nekugona kweMNP kuunganidzwa munzvimbo yakati, kuburikidza nekushandiswa kwekunze kwemagineti, kuendeswa kweanonangwa emishonga gadziriro kunovhura kusikwa kweakawanda anoshanda magineti system kunongedza cytostatics kunzvimbo yebundu. Tarisiro.Yakadaro sisitimu yaizosanganisira MNP uye magineti minda yekudzora kufamba kwavo mumuviri.Muchiitiko ichi, ese ari maviri ekunze magineti minda uye magineti implants akaiswa munzvimbo yemuviri ine bundu inogona kushandiswa seyakabva magineti.6 Nzira yekutanga ine zvikanganiso zvakakomba, zvinosanganisira kudikanwa kwekushandisa midziyo ine magineti yekunangisa zvinodhaka uye kudiwa kwekudzidzisa vashandi kuvhiya.Mukuwedzera, iyi nzira inogumira nemutengo wakakwirira uye inokodzera chete "superficial" tumors pedyo nepamusoro pemuviri.Iyo imwe nzira yekushandisa magineti implants inowedzera chiyero chekushandiswa kweiyi tekinoroji, ichifambisa kushandiswa kwayo pamamota ari munzvimbo dzakasiyana dzemuviri.Ose magineti ega ega uye magineti akasanganiswa mune intraluminal stent anogona kushandiswa seanomisikidza kukuvara kwebundu mumitezo isina maburi kuti ave nechokwadi chekubata kwavo.Zvisinei, maererano nekutsvakurudza kwedu kusina kudhindwa, izvi hazvina kukwana magineti kuti zvive nechokwadi chekuchengetedzwa kweMNP kubva muropa.
Kushanda kwemagineti kuburitsa mushonga kunoenderana nezvakawanda zvinhu: maitiro eiyo magineti inotakura pachayo, uye hunhu hwemagineti sosi (kusanganisira geometric paramita yemagineti asingagumi uye kusimba kwesimba remagineti ravanogadzira).Iko kuvandudzwa kweiyo yakabudirira magineti inotungamirwa sero inhibitor yekuendesa tekinoroji kunofanirwa kusanganisira kuvandudzwa kweakakodzera magineti nanoscale mishonga inotakura, kuongorora kuchengetedzeka kwavo, uye kugadzira yekuona protocol inobvumira kuronda mafambiro avo mumuviri.
Muchidzidzo ichi, isu takaverenga masvomhu iyo yakakwana yemagineti hunhu kudzora iyo magineti nano-chiyero chemushonga mutakuri mumuviri.Iko mukana wekuchengetedza MNP kuburikidza nemadziro etsinga yeropa pasi pesimba reyakaiswa magineti ine aya macomputer maitiro akadzidzwawo mumitsipa yeropa yemakonzo.Pamusoro pezvo, isu takagadzira conjugates eMNPs uye fluorescent agents uye takagadzira protocol yekuona kwavo mu vivo.Pasi pemamiriro ezvinhu ehupenyu, mune bundu modhi mbeva, kuunganidzwa kweMNPs mune bundu matishu painoitwa systemic pasi pesimba remagineti yakadzidzwa.
Muchidzidzo che in vitro, takashandisa referensi yeMNP, uye mune in vivo kudzidza, takashandisa MNP yakavharwa nelactic acid polyester (polylactic acid, PLA) ine fluorescent agent (indolecyanine; ICG).MNP-ICG inosanganisirwa Muchiitiko, shandisa (MNP-PLA-EDA-ICG).
Iyo synthesis uye yemuviri uye yemakemikari zvimiro zveMNP zvakatsanangurwa zvakadzama kumwe kunhu.7,8
Kuti agadzirise MNPs-ICG, PLA-ICG conjugates yakatanga kugadzirwa.Musanganiswa wepoda racemic wePLA-D uye PLA-L ine molecular uremu hwe60 kDa yakashandiswa.
Sezvo PLA neICG ese ari maviri asidhi, kuitira kuti igadzirise PLA-ICG conjugates, kutanga inoda kusanganisa amino-yakapera spacer paPLA, iyo inobatsira ICG chemisorb kune spacer.Iyo spacer yakagadziriswa uchishandisa ethylene diamine (EDA), nzira ye carbodiimide uye mvura-soluble carbodiimide, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide (EDAC).Iyo PLA-EDA spacer inogadzirwa seinotevera.Wedzera makumi maviri-yakapetwa molar yakawandisa yeEDA uye makumi maviri-yakapetwa molar yakawandisa yeEDAC kusvika ku2 mL ye0.1 g/mL PLA chloroform solution.Iyo synthesis yakaitwa mu15 mL polypropylene test chubhu pane shaker nekumhanya kwe300 min-1 kwemaawa maviri.Iyo synthesis scheme inoratidzwa muMufananidzo 1. Dzokorora kusanganiswa ne 200-fold yakawandisa reagents kuti uwedzere chirongwa chekugadzirisa.
Pakupera kweiyo synthesis, mhinduro yaive centrifuged nekumhanya kwe3000 min-1 kwemaminetsi mashanu kubvisa yakawandisa precipitated polyethylene derivatives.Zvadaro, 2 mL ye 0.5 mg/mL ICG solution mu dimethyl sulfoxide (DMSO) yakawedzerwa kune 2 mL mhinduro.Iyo agitator inogadziriswa painomhanyisa kumhanya kwe300 min-1 kwemaawa maviri.Iyo schematic dhizaini yeakawanikwa conjugate inoratidzwa muMufananidzo 2.
Mu200 mg MNP, takawedzera 4 mL PLA-EDA-ICG conjugate.Shandisa LS-220 shaker (LOIP, Russia) kumutsa kumira kwemaminetsi makumi matatu pane imwe nguva ye300 min-1.Zvadaro, yakashambidzwa ne isopropanol katatu uye yakaiswa kune magnetic separation.Shandisa UZD-2 Ultrasonic Disperser (FSUE NII TVCH, Russia) kuwedzera IPA kune kumiswa kwemaminetsi mashanu-10 pasi pekuenderera mberi kwe ultrasonic chiito.Mushure mechitatu IPA kushambidza, mvura yakashambidzwa yakashambidzwa nemvura yakasvibiswa uye yakadzorerwa muhutano hwesaline pamusangano we 2 mg / mL.
ZetaSizer Ultra midziyo (Malvern Instruments, UK) yakashandiswa kudzidza kugoverwa kwehukuru hweMNP yakawanikwa mumushonga wemvura.A transmission electron microscope (TEM) ine JEM-1400 STEM field emission cathode (JEOL, Japan) yakashandiswa kudzidza chimiro nekukura kweMNP.
Muchidzidzo ichi, tinoshandisa cylindrical anogara magineti (N35 giredhi; ine nickel inodzivirira coating) uye anotevera akajairwa saizi (kureba axis kureba × silinda dhayamita): 0.5 × 2 mm, 2 × 2 mm, 3 × 2 mm uye 5 × 2 mm.
Iyo in vitro yekudzidza yeMNP yekufambisa mune yemuenzaniso sisitimu yakaitwa pane hydrodynamic scaffold yakagadzirwa neInstitute of Experimental Medicine yeAlmazov State Medical Research Center yeRussia Ministry of Health.Huwandu hwemvura inotenderera (mvura yakasvibiswa kana Krebs-Henseleit mhinduro) ndeye 225 mL.Axially magnetized cylindrical magineti anoshandiswa semagineti echigarire.Isa magineti pachibatiso 1.5 mm kure nemadziro emukati epakati girazi chubhu, nemagumo ayo akatarisana nekwakananga kwechubhu (yakamira).Mvura inoyerera muvharo yakavharwa ndeye 60 L / h (inopindirana nemutsara we 0.225 m / s).Krebs-Henseleit solution inoshandiswa semvura inotenderera nokuti inofananidzira plasma.Iyo dynamic viscosity coefficient yeplasma ndeye 1.1-1.3 mPa∙s.9 Huwandu hweMNP yakashongedzwa mumunda wemagineti inotemerwa ne spectrophotometry kubva muhuwandu hwesimbi mumvura inotenderera mushure mekuyedza.
Pamusoro pezvo, ongororo dzekuyedza dzakaitwa patafura yakagadziridzwa yemagetsi mechanics kuona kuenzana kwemidziyo yeropa.Izvo zvikamu zvakakosha zvehutsigiro hwehydrodynamic zvinoratidzwa muMufananidzo 3. Izvo zvikamu zvikuru zve hydrodynamic stent ivhavha yakavharwa inofananidzira chikamu chemuchinjikwa wemuenzaniso vascular system uye tangi yekuchengetedza.Kufamba kweiyo modhi fluid padivi peiyo contour yeropa mudziyo module inopiwa ne peristaltic pombi.Munguva yekuyedza, chengetedza iyo vaporization uye inodiwa tembiricha renji, uye tarisa masisitimu paramita (tembiricha, kudzvanywa, kuyerera kwemvura, uye pH kukosha).
Mufananidzo 3 Chivharo dhayagiramu yekuseta inoshandiswa kudzidza kugona kweiyo carotid artery wall.1-kuchengetera tangi, 2-peristaltic pombi, 3-nzira yekuunza kumiswa ine MNP muchiuno, 4-inoyerera mita, 5-kudzvanya sensor muchiuno, 6-inopisa inotsinhanisa, 7-kamuri ine mudziyo, 8-inobva yesimba remagineti, 9-chibharumu chine mahydrocarbon.
Imba ine mudziyo ine midziyo mitatu: yekunze hombe hombe uye maviri madiki midziyo, iyo iyo maoko epakati redunhu anopfuura.Iyo cannula inopinzwa mumudziyo diki, mudziyo unosungirirwa pamudziyo diki, uye muromo wecannula wakasungwa zvakasimba netambo yakatetepa.Nzvimbo iri pakati pemudziyo mukuru uye chigadziko chiduku chinozadzwa nemvura yakasvibiswa, uye kutonhora kunoramba kuripo nekuda kwekubatanidza kune inopisa kupisa.Iyo nzvimbo mumudziyo diki yakazadzwa neKrebs-Henseleit mhinduro kuchengetedza kushanda kwemasero etsinga yeropa.Iyo tangi zvakare yakazadzwa neKrebs-Henseleit mhinduro.Iyo gasi (kabhoni) yekugovera sisitimu inoshandiswa kupfutisa mhinduro mumudziyo diki mutangi rekuchengetedza uye kamuri ine mudziyo (Mufananidzo 4).
Mufananidzo 4 Kamuri inoiswa mudziyo.1-Cannula yekudzikisa tsinga dzeropa, 2-Outer chamber, 3-Small chamber.Museve unoratidza mafambiro emvura yemuenzaniso.
Kuti uone kuenzana kwepermeability index yemadziro emudziyo, rat carotid artery yakashandiswa.
Kuiswa kweMNP kumiswa (0.5mL) muhurongwa kune maitiro anotevera: huwandu hwemukati hwetangi uye pombi yekubatanidza muchiuno ndeye 20mL, uye mukati mekamuri imwe neimwe 120mL.Iyo yekunze magineti kunobva magineti anogara ane chiyero che 2 × 3 mm.Inoiswa pamusoro peimwe yemakamuri maduku, 1 cm kure nemudziyo, uye imwe mugumo yakatarisana nemadziro emidziyo.Tembiricha inochengetwa pa37°C.Simba repombi yepombi inogadziriswa ku50%, iyo inofananidzwa nekumhanya kwe17 cm / s.Sekutonga, samples dzakatorwa muchitokisi chisina magineti echigarire.
Awa imwe chete mushure mekutonga kweiyo MNP yakapihwa, sampu yemvura yakatorwa kubva mukamuri.The particle concentration yakayerwa ne spectrophotometer uchishandisa Unico 2802S UV-Vis spectrophotometer (United Products & Instruments, USA).Tichifunga nezvekutora kweMNP kusimiswa, kuyerwa kwakaitwa pa450 nm.
Zvinoenderana nemirairo yeRus-LASA-FELASA, mhuka dzese dzinosimudzwa uye dzinosimudzwa mune chaiyo isina hutachiona nzvimbo.Chidzidzo ichi chinoenderana nemirairo yese yetsika yekuedza mhuka netsvagiridzo, uye yakawana mvumo yetsika kubva kuAlmazov National Medical Research Center (IACUC).Mhuka dzacho dzainwa mvura ad libitum uye dzaidya nguva dzose.
Chidzidzo ichi chakaitwa pagumi anesthetized anesthetized 12-week-old murume immunodeficient NSG mbeva (NOD.Cg-Prkdcscid Il2rgtm1Wjl/Szj, Jackson Laboratory, USA) 10, inorema 22 g ± 10%.Sezvo kusadzivirirwa kwe immunodeficiency mice kunodzvinyirirwa, iyo immunodeficiency mice yemutsara uyu inobvumira kutapurirana kwemasero emunhu uye matishu pasina kurambwa kwekutapurirana.Iwo ma littermates kubva kumakeji akasiyana akaiswa zvisina tsarukano kuboka rekuyedza, uye ivo vakabatanidzwa kana kurongeka pachena pamibhedha yemamwe mapoka kuti vaone kuratidzwa kwakaenzana kune yakajairika microbiota.
Iyo HeLa yekenza yekenza mutsara inoshandiswa kumisikidza xenograft modhi.Masero acho akagadzirwa muDMEM ine glutamine (PanEco, Russia), yakawedzerwa ne10% fetal bovine serum (Hyclone, USA), 100 CFU/mL penicillin, uye 100 μg/mL streptomycin.Mutsara wesero wakapiwa nemutsa neGene Expression Regulation Laboratory yeInstitute of Cell Research yeRussia Academy of Sciences.Pamberi pejekiseni, masero eHeLa aibviswa mupurasitiki yetsika ne1: 1 trypsin: Versene solution (Biolot, Russia).Mushure mekugezwa, maseru akamiswa mukati mepakati kusvika pakusangana kwe5 × 106 maseru pa200 μL, uye akacheneswa ne basement membrane matrix (LDEV-MAHARA, MATRIGEL® CORNING®) (1: 1, paaizi).Iyo yakagadzirwa sero suspension yakaiswa pasi peganda muganda regonzo chidya.Shandisa magetsi calipers kutarisa kukura kwebundu mazuva matatu ega ega.
Apo bundu rakasvika pa500 mm3, magineti echigarire akaiswa munyama yenyama yemhuka yekuedza pedyo nebundu.Muboka rekuedza (MNPs-ICG + tumour-M), 0.1 mL yeMNP kumiswa yakaiswa jekiseni uye yakaratidzwa kune magnetic field.Mhuka dzese dzisina kurapwa dzakashandiswa sekudzora (background).Pamusoro pezvo, mhuka dzakabaiwa ne 0.1 mL yeMNP asi dzisina kuisirwa magineti (MNPs-ICG + tumor-BM) dzakashandiswa.
Iyo fluorescence yekuona mu vivo uye in vitro samples yakaitwa paIVIS Lumina LT akatevedzana III bioimager (PerkinElmer Inc., USA).Kune in vitro visualization, vhoriyamu ye1 mL yekugadzira PLA-EDA-ICG uye MNP-PLA-EDA-ICG conjugate yakawedzerwa kumatsime eplate.Tichifunga nezve fluorescence hunhu hweICG dhayi, iyo yakanakisa sefa inoshandiswa kuona iyo inopenya kusimba kwemuenzaniso inosarudzwa: iyo yakanyanya excitation wavelength ndeye 745 nm, uye emission wavelength i815 nm.Iyo Living Image 4.5.5 software (PerkinElmer Inc.) yakashandiswa kuyera quantitatively kusimba kwefluorescence yematsime ane conjugate.
Iyo fluorescence intensity uye kuunganidzwa kweMNP-PLA-EDA-ICG conjugate yakayerwa mu vivo tumor modhi mbeva, pasina kuvepo uye kushandiswa kwemagineti panzvimbo yekufarira.Iro mbeva dzakaiswa anesthetized neisoflurane, uyezve 0.1 mL yeMNP-PLA-EDA-ICG conjugate yakabayiwa kuburikidza nemuswe tsinga.Mbeva dzisina kubatwa dzakashandiswa sekutonga kwakashata kuwana fluorescent background.Mushure mekuisa iyo conjugate intravenously, isa mhuka panzvimbo yekupisa (37 ° C) mukamuri yeVIS Lumina LT series III fluorescence imager (PerkinElmer Inc.) uchichengetedza inhalation ne 2% isoflurane anesthetization.Shandisa ICG's yakavakirwa-mukati sefa (745–815 nm) yekuona chiratidzo 1 miniti uye 15 maminetsi mushure mekuiswa kweMNP.
Kuongorora kuunganidzwa kwe conjugate mubundu, nzvimbo ye peritoneal yemhuka yakafukidzwa nebepa, izvo zvakaita kuti zvibvirire kubvisa kupenya kwefluorescence kwakabatana nekuunganidzwa kwezvikamu muchiropa.Mushure mekudzidza biodistribution yeMNP-PLA-EDA-ICG, mhuka dzakave dzakasimbiswa nemunhu nekudarika kwe isoflurane anesthesia kuitira kuparadzaniswa kwakatevera kwenzvimbo dzebundu uye kuongororwa kwehuwandu hwemagetsi efluorescence.Shandisa Living Image 4.5.5 software (PerkinElmer Inc.) kugadzirisa nemaoko kuongororwa kwechiratidzo kubva kunzvimbo yakasarudzwa yekufarira.Zviyero zvitatu zvakatorwa pamhuka imwe neimwe (n = 9).
Muchidzidzo ichi, hatina kuverenga kubudirira kurodha kweICG paMNPs-ICG.Mukuwedzera, isu hatina kuenzanisa kuchengetedza kwekuchengetedza kwe nanoparticles pasi pesimba remagineti asingagumi emhando dzakasiyana.Pamusoro pezvo, isu hatina kuongorora mhedzisiro yenguva refu yemagineti pakuchengetwa kwenanoparticles mumatumbu tishu.
Nanoparticles inotonga, ine avhareji saizi ye195.4 nm.Uye zvakare, iyo kumiswa yaive nemaagglomerates ane avhareji saizi ye1176.0 nm (Mufananidzo 5A).Zvadaro, chikamu chacho chakasefa kuburikidza nesefa ye centrifugal.Iyo zeta inogona yezvikamu ndeye -15.69 mV (Mufananidzo 5B).
Mufananidzo 5 Zvimiro zvemuviri zvekumiswa: (A) particle size distribution;(B) kugoverwa kwechidimbu pane zeta zvingave;(C) TEM mufananidzo we nanoparticles.
Saizi yepakati ndeye 200 nm (Mufananidzo 5C), inoumbwa neMNP imwechete ine saizi ye20 nm, uye PLA-EDA-ICG yakasanganiswa organic shell ine yakaderera electron density.Kuumbwa kweagglomerates mumhinduro dzine aqueous inogona kutsanangurwa neiyo yakaderera modulus ye electromotive simba remunhu nanoparticles.
Kune magineti echigarire, kana iyo magnetization yakanyura muvhoriyamu V, iyo yakakosha kutaura inokamurwa kuita maviri akabatanidzwa, anoti vhoriyamu uye pamusoro:
Panyaya yemuenzaniso ine magnetization inogara iripo, density iripo ndeye zero.Zvadaro, kutaura kwemagnetic induction vector kunotora fomu rinotevera:
Shandisa MATLAB chirongwa (MathWorks, Inc., USA) kuverenga nhamba, ETU "LETI" nhamba yerezinesi rezinesi 40502181.
Sezvinoratidzwa muMufananidzo 7 Mufananidzo 9 Mufananidzo-10, simba remagineti rakasimba rinogadzirwa nemagineti akaiswa axially kubva kumagumo ehumburumbira.Iyo inoshanda radius yechiito yakaenzana neiyo geometry yemagineti.Mune cylindrical magineti ane humburumbira iyo kureba kwakakura kupfuura dhayamita yayo, iyo yakasimba magineti inoonekwa muaxial-radial direction (yechikamu chinoenderana);saka, peya yemasilinda ane yakakura aspect ratio (diameter nekureba) MNP adsorption ndiyo inonyanya kushanda.
Mufananidzo we7saizi yakajairika yemagineti: mutsara mutema 0.5 × 2mm, bhuruu mutsara 2 × 2mm, girini mutsara 3 × 2mm, mutsvuku mutsetse 5 × 2mm.
Mufananidzo 8 Iyo magineti induction component Br is perpendicular to the magnet axis Oz;saizi yakajairika yemagineti: mutsara mutema 0.5 × 2mm, bhuruu mutsara 2 × 2mm, girini mutsara 3 × 2mm, mutsvuku mutsetse 5 × 2mm.
Mufananidzo 9 Iyo magineti induction intensity Bz chikamu chiri chinhambwe r kubva kumagumo axis yemagineti (z=0);saizi yakajairika yemagineti: mutsara mutema 0.5 × 2mm, bhuruu mutsara 2 × 2mm, girini mutsara 3 × 2mm, mutsvuku mutsetse 5 × 2mm.
Mufananidzo 10 Magnetic induction chikamu pamwe chete neradial direction;chiyero magineti saizi: dema mutsara 0.5 × 2mm, yebhuruu mutsara 2 × 2mm, yegirini mutsetse 3 × 2mm, mutsvuku mutsetse 5 × 2mm.
Special hydrodynamic modhi inogona kushandiswa kudzidza nzira yeMNP kuendesa kune tumor tissues, kutarisisa nanoparticles munzvimbo inotarirwa, uye kuona maitiro e nanoparticles pasi pe hydrodynamic mamiriro einotenderera system.Magineti echigarire anogona kushandiswa sekunze kwemagineti minda.Kana tikafuratira kupindirana kwemagnetostatic pakati penanoparticles uye tisingatarise iyo magineti fluid modhi, zvakakwana kufungidzira kudyidzana pakati pemagineti uye imwe nanoparticle ine dipole-dipole approximation.
Iko m ndiyo nguva yemagineti yegineti, r ndiyo radius vector yepanzvimbo iyo nanoparticle iripo, uye k ndiyo system factor.Mune dipole approximation, munda wemagineti une chimiro chakafanana (Mufananidzo 11).
Mune yunifomu yemagineti, iyo nanoparticles inotenderera chete mumitsara yesimba.Mune isina-uniform magnetic field, simba rinoshanda pairi:
Ndekupi chirevo chegwaro rakapihwa l.Mukuwedzera, simba rinokwevera nanoparticles munzvimbo dzakawanda dzisina kuenzana dzemunda, kureva, curvature uye density yemitsara yesimba inowedzera.
Naizvozvo, zvinodikanwa kushandisa magineti akasimba zvakakwana (kana magineti cheni) ine axial anisotropy iri pachena munzvimbo iyo zvidimbu zviripo.
Tafura 1 inoratidza kugona kwemagineti imwe seyakakwana magineti sosi kutora uye kuchengetedza MNP mumubhedha wevascular wendima yekushandisa.