Javascript yakadzimwa mubrowser yako parizvino. Kana javascript yakadzimwa, mamwe mabasa ewebhusaiti iyi haashande.
Nyora ruzivo rwako rwakanangana nemishonga chaiyo yaunofarira, uye tichaenzanisa ruzivo rwaunopa nezvinyorwa zviri mudhatabhesi redu rakakura uye tokutumira kopi yePDF neemail panguva yakakodzera.
Dzora kufamba kwe magnetic iron oxide nanoparticles kuti uwane cytostatics zvakananga.
Munyori Toropova Y, Korolev D, Istomina M, Shulmeyster G, Petukhov A, Mishanin V, Gorshkov A, Podyacheva E, Gareev K, Bagrov A, Demidov O
Yana Toropova,1 Dmitry Korolev,1 Maria Istomina,1,2 Galina Shulmeyster,1 Alexey Petukhov,1,3 Vladimir Mishanin,1 Andrey Gorshkov,4 Ekaterina Podyacheva,1 Kamil Gareev,2 Alexei Bagrov,5 Oleg Demidov6,71Almazov National Medical Research Center yeMinistry of Health yeRussian Federation, St. Petersburg, 197341, Russian Federation; 2 St. Petersburg Electrotechnical University “LETI”, St. Petersburg, 197376, Russian Federation; 3 Center for Personalized Medicine, Almazov State Medical Research Center, Ministry of Health yeRussian Federation, St. Petersburg, 197341, Russia Federation; 4FSBI “Influenza Research Institute yakatumidzwa zita raAA Smorodintsev” Ministry of Health yeRussian Federation, St. Petersburg, Russian Federation; 5 Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russian Federation; 6 RAS Institute of Cytology, St. Petersburg, 194064, Russian Federation; 7INSERM U1231, Faculty of Medicine and Pharmacy, Bourgogne-Franche Comté University of Dijon, France Kutaurirana: Yana ToropovaAlmazov National Medical Research Centre, Ministry of Health of the Russian Federation, Saint-Petersburg, 197341, Russian Federation Tel +7 981 95264800 4997069 Email [email protected] Nhoroondo: Nzira yakanaka yekugadzirisa dambudziko re cytostatic toxicity ndeyekushandisa magnetic nanoparticles (MNP) pakutumira mishonga. Chinangwa: Kushandisa kuverenga kuti uone hunhu hwakanakisa hwemagnetic field inodzora MNPs mumuviri, uye kuongorora kushanda kwemagnetron shipping yeMNPs kumagomarara embeva mu vitro uye mu vivo. (MNPs-ICG) inoshandiswa. Zvidzidzo zve luminescence intensity mu vivo zvakaitwa mumakonzo egomarara, ane uye asina magnetic field panzvimbo yaanofarira. Zvidzidzo izvi zvakaitwa pachigadziko che hydrodynamic chakagadzirwa neInstitute of Experimental Medicine yeAlmazov State Medical Research Center yeRussian Ministry of Health. Mugumisiro: Kushandiswa kwemagineti e neodymium kwakasimudzira kuunganidzwa kweMNP. Miniti imwe mushure mekupiwa MNPs-ICG kumakonzo ane bundu, MNPs-ICG inonyanya kuungana muchiropa. Kana pasina simba remagineti uye paine simba remagineti, izvi zvinoratidza nzira yaro yekuchinja. Kunyangwe kuwedzera kwe fluorescence mubundu kwakaonekwa kana paine simba remagineti, simba re fluorescence muchiropa chemhuka harina kuchinja nekufamba kwenguva. Mhedziso: Rudzi urwu rweMNP, pamwe chete nesimba remagineti rakaverengerwa, rinogona kuva hwaro hwekuvandudzwa kwekutumira mishonga ye cytostatic inodzorwa nemagineti kumatumbu ebundu. Mazwi akakosha: fluorescence analysis, indocyanine, iron oxide nanoparticles, magnetron delivery of cytostatics, tumor targeting
Zvirwere zvegomarara chimwe chezvikonzero zvikuru zverufu pasi rose. Panguva imwe chete, kuwedzera kwehuwandu hwezvirwere nekufa kwezvirwere zvegomarara kuchiripo. 1 Mishonga yekurapa kenza inoshandiswa nhasi ichiri imwe yenzira huru dzekurapa magomarara akasiyana. Panguva imwe chete, kugadzira nzira dzekuderedza chepfu ye cytostatics kuchiri kukosha. Nzira inovimbisa yekugadzirisa dambudziko rayo rechepfu ndeyekushandisa nzira dzekutakura mishonga, idzo dzinogona kupa huwandu hwemishonga munzvimbo dzegomarara pasina kuwedzera huwandu hwayo munhengo dzine hutano nenyama. 2 Nzira iyi inoita kuti zvikwanisike kuvandudza kushanda uye kunangwa kwemishonga yekurapa kenza pamatishu egomarara, ukuwo ichideredza chepfu yayo yemuviri.
Pakati pezvikamu zvakasiyana-siyana zve nanoparticles zvinofungidzirwa kuti zviendeswe kune vanhu vane cytostatic agents, magnetic nanoparticles (MNPs) zvinonyanya kufarirwa nekuda kwehunhu hwazvo hwemakemikari, zvipenyu, uye magnetic, izvo zvinoita kuti zvive nyore kushandisa. Nokudaro, magnetic nanoparticles inogona kushandiswa senzira yekudziyisa kurapa bundu rine hyperthermia (magnetic hyperthermia). Inogonawo kushandiswa senzira dzekuongorora (magnetic resonance diagnosis). 3-5 Uchishandisa hunhu uhwu, pamwe chete nemukana wekuungana kweMNP munzvimbo yakati, kuburikidza nekushandisa simba rekunze remagineti, kuendesa mishonga yakanangwa kunovhura kugadzirwa kwesimba remagineti rinoshanda zvakasiyana-siyana kuti ritarise cytostatics kunzvimbo yebundu. Sisitimu yakadaro inosanganisira MNP neminda yemagetsi kudzora kufamba kwavo mumuviri. Muchiitiko ichi, minda yemagetsi yekunze uye magnetic implants yakaiswa munzvimbo yemuviri ine bundu zvinogona kushandiswa senzvimbo yesimba remagineti. 6 Nzira yekutanga ine zvikanganiso zvakakomba, kusanganisira kudiwa kwekushandisa michina yakasarudzika yekutarisa mishonga ine magnetic uye kudiwa kwekudzidzisa vashandi kuita kuvhiya. Pamusoro pezvo, nzira iyi inogumira pamutengo wakakwira uye yakakodzera chete kumabundu "epamusoro" ari pedyo nepamusoro pemuviri. Imwe nzira yekushandisa magineti implants inowedzera huwandu hwekushandiswa kwetekinoroji iyi, zvichiita kuti ishandiswe pamabundu ari munzvimbo dzakasiyana dzemuviri. Magineti ega ega nemagineti akabatanidzwa mu intraluminal stent anogona kushandiswa semagineti ekukuvara kwebundu munhengo dzisina chinhu kuti ave nechokwadi chekuti akasimba. Zvisinei, zvichienderana nekutsvagurudza kwedu kusina kuburitswa, aya haana magineti akakwana kuti ave nechokwadi chekuti MNP inochengetwa kubva muropa.
Kushanda kwekushandisa magnetron kunoenderana nezvinhu zvakawanda: hunhu hwemuchina unotakura magnetic pachawo, uye hunhu hwesimba remagnetic field source (kusanganisira ma geometric parameters emagineti anogara aripo uye simba resimba remagnetic field raanogadzira). Kugadzirwa kwetekinoroji yekubudirira yekuendesa magnetically guided cell inhibitor inofanira kusanganisira kugadzirwa kwemuchina unotakura magnetic nanoscale wakakodzera, kuongorora kuchengetedzeka kwawo, uye kugadzira nzira yekuona inobvumidza kuteedzera mafambiro awo mumuviri.
Muchidzidzo ichi, takaverenga nemasvomhu hunhu hwesimba remagineti hwakakodzera kudzora chinotakura mushonga wemagnetic nano-scale mumuviri. Mikana yekuchengetedza MNP kuburikidza nemadziro etsinga yeropa pasi pesimba remagineti rakashandiswa rine hunhu uhwu hwekuverenga yakadzidzwawo mutsinga dzeropa dzemakonzo dzakaparadzana. Pamusoro pezvo, takagadzira ma conjugates eMNPs uye fluorescent agents uye takagadzira nzira yekuona kwavo mumuviri. Pasi pemamiriro ezvinhu emuviri, mumbeva dzemhando yegomarara, kushanda zvakanaka kweMNPs mumatishu egomarara kana ikashandiswa nenzira yemuviri pasi pesimba remagineti kwakadzidzwa.
Muchidzidzo che in vitro, takashandisa reference MNP, uye muchidzidzo che in vivo, takashandisa MNP yakaputirwa ne lactic acid polyester (polylactic acid, PLA) ine fluorescent agent (indolecyanine; ICG). MNP-ICG inosanganisirwa muChidzidzo che, shandisa (MNP-PLA-EDA-ICG).
Kugadzirwa kweMNP, hunhu hwemuviri nehwemakemikari zvakatsanangurwa zvakadzama mune dzimwe nzvimbo.
Kuti MNPs-ICG igadziriswe, maPLA-ICG conjugates akatanga kugadzirwa. Musanganiswa wePLA-D nePLA-L une huremu hwe60 kDa wakashandiswa.
Sezvo PLA neICG zviri maasidhi ese, kuti zvigadzire maPLA-ICG conjugates, zvinofanirwa kutanga zvagadzira amino-terminated spacer paPLA, iyo inobatsira ICG chemisorb kuenda kuspacer. Spacer yakagadzirwa uchishandisa ethylene diamine (EDA), carbodiimide method uye water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC). PLA-EDA spacer yakagadzirwa seinotevera. Wedzera 20-fold molar excess yeEDA uye 20-fold molar excess yeEDAC kune 2 mL ye 0.1 g/mL PLA chloroform solution. Kugadzira kwakaitwa mu 15 mL polypropylene test tube pa shaker nekumhanya kwe 300 min-1 kwemaawa maviri. Kugadzira kwacho kunoratidzwa muMufananidzo 1. Dzokorora kugadzira kwacho ne 200-fold excess ye reagents kuti ugadzirise synthesis scheme.
Pakupera kwekugadzirwa, mushonga wacho wakaiswa pakati pemvura nekumhanya kwe3000 min-1 kwemaminitsi mashanu kuti ubvise zvinhu zvakasara zve polyethylene. Zvadaro, 2 mL ye 0.5 mg/mL ICG solution mu dimethyl sulfoxide (DMSO) yakawedzerwa kune 2 mL solution. Agitator inogadziriswa nekumhanya kwekukurunga kwe300 min-1 kwemaawa maviri. Schematic diagram ye conjugate yakawanikwa inoratidzwa muMufananidzo 2.
Mu 200 mg MNP, takawedzera 4 mL PLA-EDA-ICG conjugate. Shandisa LS-220 shaker (LOIP, Russia) kuti ukurumidze suspension kwemaminitsi makumi matatu pa frequency ye 300 min-1. Zvadaro, yakagezwa ne isopropanol katatu uye yakaiswa magnetic separation. Shandisa UZD-2 Ultrasonic Disperser (FSUE NII TVCH, Russia) kuwedzera IPA pa suspension kwemaminitsi mashanu kusvika gumi pasi pe ultrasonic action inoenderera mberi. Mushure mekushambidzwa kwechitatu kwe IPA, precipitate yakagezwa nemvura yakasanganiswa uye yakadzorerwa mu physiological saline pa concentration ye 2 mg/mL.
Midziyo yeZetaSizer Ultra (Malvern Instruments, UK) yakashandiswa kudzidza kugoverwa kwehukuru hweMNP yakawanikwa mumvura ine mvura. Microscope yekutumira maerekitironi (TEM) ine JEM-1400 STEM field emission cathode (JEOL, Japan) yakashandiswa kudzidza chimiro nehukuru hweMNP.
Muchidzidzo chino, tinoshandisa magineti anogara ari cylindrical (N35 grade; ane nickel protective coating) uye saizi dzakajairwa dzinotevera (long axis length × cylinder diameter): 0.5×2 mm, 2×2 mm, 3×2 mm uye 5×2 mm.
Chidzidzo chekufambisa MNP muchimiro chemuenzaniso chakaitwa pachikuva che hydrodynamic chakagadzirwa neInstitute of Experimental Medicine yeAlmazov State Medical Research Center yeRussian Ministry of Health. Huwandu hwemvura inotenderera (mvura yakanyungudutswa kana mhinduro yeKrebs-Henseleit) i225 mL. Magneti e cylindrical ane magnetized anoshandiswa semagineti anogara aripo. Isa magineti pachibatiso 1.5 mm kubva pamadziro emukati mechubhu yegirazi yepakati, mugumo wayo wakatarisana negwara rechubhu (yakatwasuka). Mwero wekuyerera kwemvura muchubhu yakavharwa i60 L/h (inoenderana ne linear velocity ye 0.225 m/s). Mhinduro yeKrebs-Henseleit inoshandiswa semvura inotenderera nekuti inofanana neplasma. Dynamic viscosity coefficient yeplasma i1.1–1.3 mPa∙s. 9 Huwandu hweMNP hwakanyungudutswa mumunda wemagineti hunotsanangurwa ne spectrophotometry kubva pakuwanda kwesimbi mumvura inotenderera mushure mekuedza.
Pamusoro pezvo, zvidzidzo zvekuyedza zvakaitwa patafura yemakanika emvura yakagadziridzwa kuti vaone kupindirana kwetsinga dzeropa. Zvikamu zvikuru zvekutsigira kwehydrodynamic zvinoratidzwa muMufananidzo 3. Zvikamu zvikuru zve hydrodynamic stent idenderedzwa rakavharwa rinotevedzera chikamu chemugero we vascular system yemuenzaniso uye tangi rekuchengetera. Kufamba kwemvura yemuenzaniso pamwe chete ne contour ye blood vessel module kunopihwa ne peristaltic pombi. Munguva yekuedza, chengetedza vaporization uye tembiricha inodiwa, uye tarisa ma parameter esystem (tembiricha, kumanikidzwa, mwero wekuyerera kwemvura, uye pH value).
Mufananidzo 3 Dhayagiramu yebhuroko remuchina unoshandiswa kudzidza kupenya kwemadziro etsinga ye carotid. Tangi rekuchengetera 1, pombi yeperistaltic 2, nzira nhatu dzekuisa suspension ine MNP mu loop, 4-flow meter, 5-pressure sensor mu loop, 6-heat exchanger, 7-chamber ine container, 8-source yemagnetic field, 9-bharuni rine hydrocarbons.
Kamuri rine mudziyo uyu rine midziyo mitatu: mudziyo mukuru wekunze nemidziyo miviri midiki, umo maoko edunhu repakati anopinda. Cannula inopinzwa mumudziyo mudiki, mudziyo wacho unosungirirwa pamudziyo mudiki, uye muromo wecannula wakasungwa zvakasimba newaya yakatetepa. Nzvimbo iri pakati pemudziyo mukuru nemudziyo mudiki inozadzwa nemvura yakasanganiswa, uye tembiricha inoramba iripo nekuda kwekubatana neheat exchanger. Nzvimbo iri mumudziyo mudiki inozadzwa neKrebs-Henseleit solution kuchengetedza kushanda kwemasero eropa. Tangi rinozadzwawo neKrebs-Henseleit solution. Sisitimu yekupa gasi (carbon) inoshandiswa kupfavisa mhinduro iri mumudziyo mudiki mutangi rekuchengetera nekamuri rine mudziyo (Mufananidzo 4).
Mufananidzo 4 Kamuri rinoiswa mudziyo. 1-Cannula yekudzikisa mitsipa yeropa, 2-Kamuri rekunze, 3-Kamuri diki. Museve unoratidza divi remvura yemuenzaniso.
Kuti vaone kana madziro etsinga ari kuyerera zvakanaka, tsinga yeropa inonzi carotid artery yakashandiswa.
Kuiswa kweMNP suspension (0.5mL) musystem kune hunhu hunotevera: huwandu hwese hwemukati metangi nepombi inobatanidza mudenderedzwa i20mL, uye huwandu hwemukati mekamuri yega yega i120mL. Soko rekunze remagineti imagneti inogara iripo ine saizi yakajairika ye2×3 mm. Yakaiswa pamusoro peimwe yemakamuri madiki, 1 cm kubva mugaba, nemugumo mumwe wakatarisana nemadziro egaba. Tembiricha inochengetwa pa37°C. Simba repombi inotenderera rakaiswa pa50%, iyo inoenderana nekumhanya kwe17 cm/s. Sekutonga, sampuro dzakatorwa musero isina magineti anogara aripo.
Awa imwe mushure mekupiwa kwehuwandu hwakati wandei hweMNP, sampuro yemvura yakatorwa kubva mukamuri. Huwandu hwezvinhu zvakakamurwa ne spectrophotometer uchishandisa Unico 2802S UV-Vis spectrophotometer (United Products & Instruments, USA). Tichifunga nezve absorption spectrum yeMNP suspension, kuyerwa kwacho kwakaitwa pa450 nm.
Zvichienderana nemirairo yeRus-LASA-FELASA, mhuka dzese dzinorerwa uye dzinorerwa munzvimbo dzisina utachiona. Chidzidzo ichi chinotevedzera mitemo yese yetsika yekuedza nekutsvaga mhuka, uye chakawana mvumo yetsika kubva kuAlmazov National Medical Research Center (IACUC). Mhuka idzi dzainwa mvura nguva dzese uye dzaipihwa chikafu nguva nenguva.
Chidzidzo ichi chakaitwa pambeva gumi dzechirume dzeNSG dzine mavhiki gumi nemaviri dzakarapwa nekurwadziwa (NOD.Cg-Prkdcscid Il2rgtm1Wjl/Szj, Jackson Laboratory, USA) 10, dzine huremu hwe22 g ± 10%. Sezvo dziviriro yembeva dzekudzivirira dziviriro yemuviri yakaderedzwa, mbeva dzerudzi urwu dzinobvumira kuiswa kwemasero nematishu evanhu pasina kurambwa kuiswa kwemuviri. Mbeva dzakabva mumakeji akasiyana dzakaiswa muboka rekuyedza, uye dzakaberekeswa pamwe chete kana kuiswa pachena nenzira yakarongwa pamubhedha wemamwe mapoka kuti dzive nechokwadi chekuti dzine utachiona hwakaenzana nehutachiona hwemuviri.
Mutsetse wemasero ekenza yevanhu weHeLa unoshandiswa kugadzira muenzaniso wexenograft. Masero aya akakurirwa muDMEM ine glutamine (PanEco, Russia), yakawedzerwa 10% fetal bovine serum (Hyclone, USA), 100 CFU/mL penicillin, uye 100 μg/mL streptomycin. Mutsetse wemasero wakapihwa nemutsa neGene Expression Regulation Laboratory yeInstitute of Cell Research yeRussian Academy of Sciences. Vasati vabaya jekiseni, masero eHeLa akabviswa mupurasitiki yekukura ne1:1 trypsin:Versene solution (Biolot, Russia). Mushure mekusukwa, masero akasungirirwa pakati nepakati kusvika pakuwanda kwemasero 5×106 pa 200 μL, uye akasanganiswa ne basement membrane matrix (LDEV-FREE, MATRIGEL® CORNING®) (1:1, pachando). Kusungirirwa kwemasero kwakagadzirwa kwakabayiwa pasi peganda remuhudyu wembeva. Shandisa ma electronic calipers kutarisa kukura kwebundu mazuva ese matatu.
Bundu parakasvika 500 mm3, magineti inogara iripo yakaiswa mutsandanyama yemhuka yekuedza iri pedyo nebundu. Muboka rekuyedza (MNPs-ICG + tumor-M), 0.1 mL yeMNP suspension yakabayiwa uye yakaiswa panzvimbo ine magineti. Mhuka dzese dzisina kurapwa dzakashandiswa senzira dzekudzora (background). Pamusoro pezvo, mhuka dzakabayiwa 0.1 mL yeMNP asi dzisina kuiswa magineti (MNPs-ICG + tumor-BM) dzakashandiswa.
Kuonekwa kwechiedza che fluorescence che samples dze in vivo ne in vitro kwakaitwa pa IVIS Lumina LT series III bioimager (PerkinElmer Inc., USA). Pakuona kwechiedza che in vitro, vhoriyamu ye 1 mL ye synthetic PLA-EDA-ICG ne MNP-PLA-EDA-ICG conjugate yakawedzerwa kuma plate wells. Tichifunga nezve fluorescence characteristics ye ICG dye, filter yakanakisa inoshandiswa kuona luminous intensity ye sample inosarudzwa: maximum excitation wavelength i 745 nm, uye emission wavelength i 815 nm. Software ye Living Image 4.5.5 (PerkinElmer Inc.) yakashandiswa kuyera quantitatively fluorescence intensity ye ma wells ane conjugate.
Kuwanda kwechiedza chechiedza uye kuunganidzwa kweMNP-PLA-EDA-ICG conjugate zvakayerwa mumbeva dzemhando yegomarara remuviri, pasina kuvapo uye kushandiswa kwesimba remagineti panzvimbo yaidiwa. Mbeva dzakapihwa mushonga wekurapa neisoflurane, uye 0.1 mL yeMNP-PLA-EDA-ICG conjugate yakabayiwa kuburikidza netsinga yemuswe. Mbeva dzisina kurapwa dzakashandiswa senzira yekudzivirira kuti dzionekwe nechiedza chechiedza. Mushure mekupa mushonga wekudzivirira chirwere netsinga, isa mhuka panzvimbo yekupisa (37°C) mukamuri reIVIS Lumina LT series III fluorescence imager (PerkinElmer Inc.) uku uchiramba uchifema ne2% isoflurane anaesthetization. Shandisa firita yakavakirwa mukati meICG (745–815 nm) kuti uzive zviratidzo kwemaminitsi 1 nemaminitsi 15 mushure mekuisirwa MNP.
Kuti vaongorore kuungana kwe conjugate mubundu, nzvimbo yeperitoneal yemhuka yakafukidzwa nepepa, izvo zvakaita kuti zvibviswe kubvisa kupenya kwakajeka kwakabatana nekuungana kwezvidimbu muchiropa. Mushure mekudzidza kugoverwa kweMNP-PLA-EDA-ICG, mhuka dzakaurayiwa neropa nekuwedzerwa kwe isoflurane anesthesia kuti nzvimbo dzebundu dziparadzaniswe uye kuongororwa kwehuwandu hwemwaranzi ye fluorescence ionekwe. Shandisa software yeLiving Image 4.5.5 (PerkinElmer Inc.) kuti ugadzirise ongororo yechiratidzo kubva munzvimbo yakasarudzwa. Zviyero zvitatu zvakatorwa pamhuka yega yega (n = 9).
Muchidzidzo ichi, hatina kuverenga huwandu hwe ICG yakabudirira kurodha pa MNPs-ICG. Pamusoro pezvo, hatina kuenzanisa kushanda zvakanaka kwe nanoparticles pasi pesimba remagineti anogara aripo emhando dzakasiyana. Pamusoro pezvo, hatina kuongorora mhedzisiro yenguva refu yesimba remagineti pakuchengetedza nanoparticles mumatishu egomarara.
Nanoparticles ndiyo inonyanya kuoneka, ine avhareji yehukuru hwe195.4 nm. Pamusoro pezvo, suspension yacho yaive nema agglomerates ane avhareji yehukuru hwe1176.0 nm (Mufananidzo 5A). Zvadaro, chikamu chacho chakaseferwa kuburikidza ne centrifugal filter. Zeta potential yezvikamu izvi ndeye -15.69 mV (Mufananidzo 5B).
Mufananidzo 5 Hunhu hwemuviri hwekumiswa: (A) kugoverwa kwehukuru hwezvikamu; (B) kugoverwa kwezvikamu pa zeta potential; (C) Mufananidzo weTEM wezvikamu zvidiki.
Saizi yezvikamu izvi inenge iri 200 nm (Mufananidzo 5C), inoumbwa neMNP imwe chete ine saizi ye20 nm, uye ganda rePLA-EDA-ICG rakabatana rine density yakaderera yemaerekitironi. Kuumbwa kwema agglomerates mumvura kunogona kutsanangurwa ne modulus yakaderera yesimba remagetsi re nanoparticles imwe neimwe.
Kune magineti anogara aripo, kana magnetization yakabatanidzwa muvhoriyamu V, kutaura kwe integral kunokamurwa kuita ma integral maviri, kureva vhoriyamu nepamusoro:
Kana iri sample ine constant magnetization, current density is zero. Zvadaro, kutaura kwe magnetic induction vector kuchaita seizvi:
Shandisa chirongwa cheMATLAB (MathWorks, Inc., USA) pakuverenga nhamba, ETU "LETI" academic license number 40502181.
Sezvakaratidzwa muMufananidzo 7 Mufananidzo 8 Mufananidzo 9 Mufananidzo-10, simba remagineti rakasimba rinogadzirwa nemagnet akatarisa axially kubva kumagumo esilinda. Radius inoshanda yekushanda yakaenzana nejometri yemagineti. Mumagineti e cylindrical ane silinda ine kureba kwakakura kupfuura dhayamita yayo, simba remagineti rakasimba rinoonekwa munzira ye axial-radial (yechikamu chinoenderana); saka, ma cylinders maviri ane aspect ratio yakakura (dhayamita nehurefu) MNP adsorption ndiyo inonyanya kushanda.
Mufananidzo 7 Chikamu chesimba remagineti reBz pamwe neOz axis yemagineti; saizi yakajairika yemagineti: mutsetse mutema 0.5×2mm, mutsetse webhuruu 2×2mm, mutsetse wegirini 3×2mm, mutsetse mutsvuku 5×2mm.
Mufananidzo 8 Chikamu che magnetic induction Br chakamira pa magnet axis Oz; saizi yakajairika yemagineti: mutsetse mutema 0.5×2mm, mutsetse webhuruu 2×2mm, mutsetse wegirini 3×2mm, mutsetse mutsvuku 5×2mm.
Mufananidzo 9 Chikamu cheBz chinopinza simba remagineti chiri kure ne r kubva kumagumo egineti (z=0); saizi yakajairika yemagineti: mutsetse mutema 0.5×2mm, mutsetse webhuruu 2×2mm, mutsetse wegirini 3×2mm, mutsetse mutsvuku 5×2mm.
Mufananidzo 10 Chikamu chemagnetic induction chiri munzira ye radial; saizi yakajairika yemagineti: mutsetse mutema 0.5×2mm, mutsetse webhuruu 2×2mm, mutsetse wegirini 3×2mm, mutsetse mutsvuku 5×2mm.
Mamodheru ehydrodynamic akakosha anogona kushandiswa kudzidza nzira yekuendesa MNP kumatishu egomarara, kuisa nanoparticles munzvimbo yakanangwa, uye kuona maitiro e nanoparticles pasi pemamiriro ehydrodynamic mu circulatory system. Magnets anogara aripo anogona kushandiswa senzvimbo dzekunze dzemagineti. Kana tikasateerera kutaurirana kwemagnetostatic pakati pe nanoparticles uye tisingafunge nezve magnetic fluid model, zvakakwana kufungidzira kudyidzana kuripo pakati pemagineti ne nanoparticle imwe chete ine dipole-dipole approximation.
Apo m iri nguva yemagineti yemagineti, r ndiyo radius vector yenzvimbo ine nanoparticle, uye k ndiyo system factor. Muchidimbu che dipole, munda wemagineti une configuration yakafanana (Mufananidzo 11).
Mumunda wemagineti wakaenzana, zvimedu zvidiki zvinongotenderera chete zvichienderana nemitsara yesimba. Mumunda wemagineti usina kufanana, simba rinoshanda pairi:
Chinobva kune imwe nzira l chiri kupi. Pamusoro pezvo, simba rinodhonza nanoparticles munzvimbo dzisina kuenzana mumunda, kureva kuti, kutenderera uye kuwanda kwemitsara yesimba kunowedzera.
Saka, zvinokurudzirwa kushandisa magineti akasimba zvakakwana (kana cheni yemagineti) ane axial anisotropy inooneka munzvimbo ine zvidimbu zvacho.
Tafura 1 inoratidza kugona kwemagineti rimwe chete senzvimbo yakakwana yemagineti yekubata nekuchengetedza MNP mumitsetse yetsinga yemunda wekushandisa.